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	Provedor de dados ArchiMer (2) Autor Colwell, Rita (2) Hervio Heath, Dominique (2) Pommepuy, Monique (2) Coutard, Francois (1) Crassous, Philippe (1) Droguet, Michael (1) Fischer Le Saux, Marion (1) Gobin, Eric (1) Loaec, Solen (1) Mais... Palavra-chave Artificial seawater (1) Cytotoxin Hemolysin (1) Molecular detection method (1) Regrowth (1) Resuscitation (1) Reverse transcription PCR (1) Scanning electron microscopy (1) Transmission electron microscopy (1) VBNC state (1) Vibrio parahaemolyticus (1) Vibrio vulnificus (1) Mais... Tipo do documento Text (2) Ano 2007 (1) 2002 (1) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater ArchiMer Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique. The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when... Tipo: Text Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus. Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation? ArchiMer Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique. The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population... Tipo: Text Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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